Oxidative Phosphorylation by Subcellular Particles from Cabbage

Factors affecting oxidative phosphorylation by subcellular particles isolated from cotton seedling hypocotyls.

Properly prepared mitochondrial fractions from plant sources are capable of incorporating inorganic phosphate into high energy bonds upon the oxidation of members of the tricarboxylic acid cycle (3, 6, 7). Oxidative phosphorylation has been demonstrated in preparations from a wide variety of plant sources. This author reported that a high concentration of BSA3 in the grinding medium was necessa...

Intermediate reactions of oxidative phosphorylation in mitochondria from cabbage.

Respiratory control ratios between 2.0 and 9.0 were obtained by comparison of the respiratory rates of cabbage mitochondria in the presence and in the absence of individual components of the system used to provide ADP and by comparing the rates before and after exhaustion of added ADP. These results indicate that respiration in cabbage mitochondria is controlled by the availability of ADP, whic...

Oxidative Phosphorylation and Glycolate Oxidation by Particles from Spinach Leaves.

It has recently been reported (2) that oxidative phosphorylation occurs when organic acids of the citric acid cycle are supplied as substrates to particles isolated from the cotyledons of etiolated lupine seedlings. The ratios of the number of moles of orthophosphate consumed to the number of atoms of oxygen taken up (P/0 ratios) were found to be as high as those previously reported only for an...

Oxidative phosphorylation catalyzed by cytoplasmic particles isolated from malignant tissues.

Interaction of fluorescent probes with submitochondrial particles during oxidative phosphorylation.

Addition of several N-arylaminonaphthalenesulfonate dyes to submitochondrial particles from bovine heart resulted in an enhancement of fluorescence of from SOto 150-fold and a considerable shift in the wave length of maximum emission toward the ultraviolet. The results indicate 40 to 55 dyebinding sites per mg of submitochondrial particles. A preliminary search revealed that mitochondrial ATPas...